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vector plasmids pcdna3 bap sox2  (Addgene inc)


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    Addgene inc vector plasmids pcdna3 bap sox2
    Vector Plasmids Pcdna3 Bap Sox2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+plasmids+pcdna3+bap+sox2/pcDNA3-BAP-Sox2+(Plasmid+%23133281)/pmc09862934-37-1-9
    Average 93 stars, based on 3 article reviews
    vector plasmids pcdna3 bap sox2 - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR
    Article Snippet: The vector plasmids pcDNA3-BAP-SOX2 and pOz-humBirA-GFP are available from Addgene (Addgene ID 133281 and 133283, respectively).

    Article Title: Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR.
    Article Snippet: The vector plasmids pcDNA3-BAP-SOX2 and pOz-humBirA-GFP are available from Addgene (Addgene ID 133281 and 133283, respectively).

    Article Title: In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2 and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation
    Article Snippet: The vector plasmids pcDNA3-BAP-Sox2 and pOz-humBirA-GFP are available from Addgene (Addgene ID 133281 and 133283, respectively).



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    vector plasmids pcdna3 bap sox2  (Addgene inc)
    93
    Addgene inc vector plasmids pcdna3 bap sox2
    Vector Plasmids Pcdna3 Bap Sox2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+plasmids+pcdna3+bap+sox2/pcDNA3-BAP-Sox2+(Plasmid+%23133281)/pmc09862934-37-1-9
    Average 93 stars, based on 1 article reviews
    vector plasmids pcdna3 bap sox2 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    vector plasmids pcdna3 bap1070 sox2  (Addgene inc)
    93
    Addgene inc vector plasmids pcdna3 bap1070 sox2
    Validation of the interaction of BAP-X and BirA-Y. ( A ) Experimental workflow for the detection of protein–protein interactions between <t>SOX2</t> and OCT4. ( B ) Western blots of the experiment where X = SOX2 and Y = OCT4. The positions of BAP-fusions and nonspecific signals (NS) are indicated by asterisks. The biotin labeling time was 3 h. The BAP fragment has a His-tag, and the first western blot shows comparable total amounts of BAP-GFP and BAP-SOX2. The Streptavidin blot shows the number of biotin-labeled BAP-fusions which are the result of protein–protein interactions (or proximity) of BAP-SOX2 and BirA-OCT4 in lane 2. A very strong signal was observed in lane 2, and a very weak signal was observed in lane 1, which may be the result of a random collision of BAP-GFP and BirA-OCT4. Treatment with an anti-SOX2 antibody on the third blot indicates recombinant BAP-SOX2 and the absence of a detectable amount of endogenous SOX2. BAP adds a mass shift of 3 kDa, and only a single band was observed on the anti-SOX2 blot in lane 2. ( C ) Western blots of the reciprocal experiment where X = OCT4 and Y = SOX2. The biotin labeling time was 6 h. A comparable expression of BAP-GFP and BAP-OCT4 was detected on anti-His blot lanes 3 and 4. The left blots show the expression, the middle blots show the interaction and the right blots show the absence of the endogenous proteins SOX2 or OCT4. Note that BirA-X (which is actually BirA-8×His-tag-X) fusions were not observed on anti-His blots.
    Vector Plasmids Pcdna3 Bap1070 Sox2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+plasmids+pcdna3+bap+sox2/pcDNA3-BAP-Sox2+(Plasmid+%23133281)/pmc09862934-177-1-10
    Average 93 stars, based on 1 article reviews
    vector plasmids pcdna3 bap1070 sox2 - by Bioz Stars, 2026-10
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    Validation of the interaction of BAP-X and BirA-Y. ( A ) Experimental workflow for the detection of protein–protein interactions between SOX2 and OCT4. ( B ) Western blots of the experiment where X = SOX2 and Y = OCT4. The positions of BAP-fusions and nonspecific signals (NS) are indicated by asterisks. The biotin labeling time was 3 h. The BAP fragment has a His-tag, and the first western blot shows comparable total amounts of BAP-GFP and BAP-SOX2. The Streptavidin blot shows the number of biotin-labeled BAP-fusions which are the result of protein–protein interactions (or proximity) of BAP-SOX2 and BirA-OCT4 in lane 2. A very strong signal was observed in lane 2, and a very weak signal was observed in lane 1, which may be the result of a random collision of BAP-GFP and BirA-OCT4. Treatment with an anti-SOX2 antibody on the third blot indicates recombinant BAP-SOX2 and the absence of a detectable amount of endogenous SOX2. BAP adds a mass shift of 3 kDa, and only a single band was observed on the anti-SOX2 blot in lane 2. ( C ) Western blots of the reciprocal experiment where X = OCT4 and Y = SOX2. The biotin labeling time was 6 h. A comparable expression of BAP-GFP and BAP-OCT4 was detected on anti-His blot lanes 3 and 4. The left blots show the expression, the middle blots show the interaction and the right blots show the absence of the endogenous proteins SOX2 or OCT4. Note that BirA-X (which is actually BirA-8×His-tag-X) fusions were not observed on anti-His blots.

    Journal: Life

    Article Title: Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR

    doi: 10.3390/life13010107

    Figure Lengend Snippet: Validation of the interaction of BAP-X and BirA-Y. ( A ) Experimental workflow for the detection of protein–protein interactions between SOX2 and OCT4. ( B ) Western blots of the experiment where X = SOX2 and Y = OCT4. The positions of BAP-fusions and nonspecific signals (NS) are indicated by asterisks. The biotin labeling time was 3 h. The BAP fragment has a His-tag, and the first western blot shows comparable total amounts of BAP-GFP and BAP-SOX2. The Streptavidin blot shows the number of biotin-labeled BAP-fusions which are the result of protein–protein interactions (or proximity) of BAP-SOX2 and BirA-OCT4 in lane 2. A very strong signal was observed in lane 2, and a very weak signal was observed in lane 1, which may be the result of a random collision of BAP-GFP and BirA-OCT4. Treatment with an anti-SOX2 antibody on the third blot indicates recombinant BAP-SOX2 and the absence of a detectable amount of endogenous SOX2. BAP adds a mass shift of 3 kDa, and only a single band was observed on the anti-SOX2 blot in lane 2. ( C ) Western blots of the reciprocal experiment where X = OCT4 and Y = SOX2. The biotin labeling time was 6 h. A comparable expression of BAP-GFP and BAP-OCT4 was detected on anti-His blot lanes 3 and 4. The left blots show the expression, the middle blots show the interaction and the right blots show the absence of the endogenous proteins SOX2 or OCT4. Note that BirA-X (which is actually BirA-8×His-tag-X) fusions were not observed on anti-His blots.

    Article Snippet: The vector plasmids pcDNA3-BAP1070-SOX2 and pOz-BirA-GFP were deposited in the Addgene repository (ID 133281 and 133283).

    Techniques: Western Blot, Labeling, Recombinant, Expressing

    Real-time qRT-PCR amplification of OCT4 and SOX2 in the PUB experiments. Error bars represent the standard deviation.

    Journal: Life

    Article Title: Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR

    doi: 10.3390/life13010107

    Figure Lengend Snippet: Real-time qRT-PCR amplification of OCT4 and SOX2 in the PUB experiments. Error bars represent the standard deviation.

    Article Snippet: The vector plasmids pcDNA3-BAP1070-SOX2 and pOz-BirA-GFP were deposited in the Addgene repository (ID 133281 and 133283).

    Techniques: Quantitative RT-PCR, Amplification, Standard Deviation